Kayayyaki
Kaji Cutar Bursal Cutar Cutar Ab Elisa Kit
Kaji Cutar Bursal Cutar Cutar Ab Elisa Kit
| Takaitawa | Gano kawar da maganin rigakafi daga cutar bursa na Fabricius virus a cikin maganin kaji |
| Abubuwan Ganewa | Chicken kamuwa da cutar bursal virus antibody |
| Misali | Magani
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| Yawan | 1 kit = 192 Gwaji |
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Kwanciyar hankali da Ajiya | 1) Duk reagents ya kamata a adana a 2 ~ 8 ℃.Kar a daskare. 2) Rayuwar rayuwa wata 12 ce.Yi amfani da duk reagents kafin ranar karewa akan kit.
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Bayani
Cutar bursal mai kamuwa da cuta(IBD), wanda kuma aka fi sani da cutar Gumboro, cututtukan bursitis da cutar nephrosis na Avian, cuta ce mai saurin yaduwa ta matasa.kajida kuma turkeys da ke haifar da ƙwayar cuta mai saurin kamuwa da cuta (IBDV), wanda aka kwatanta da suimmunosuppressionda mace-mace gabaɗaya a cikin makonni 3 zuwa 6.An fara gano cutar a cikiGumboro, Delawarea cikin 1962. Yana da mahimmancin tattalin arziki ga masana'antar kiwon kaji a duk duniya saboda karuwar kamuwa da wasu cututtuka da kuma tsangwama mara kyau tare da tasiri.maganin alurar riga kafi.A cikin 'yan shekarun nan, nau'ikan nau'ikan IBDV (vvIBDV), suna haifar da mummunar mace-mace a cikin kaji, sun bayyana a Turai,Latin Amurka,Kudu-maso Gabashin Asiya, Afirka da kumaGabas ta Tsakiya.Kamuwa da cuta yana ta hanyar oro-fecal, tare da tsuntsun da ya shafa yana fitar da babban matakan ƙwayar cuta na kusan makonni 2 bayan kamuwa da cuta.Cutar na yaduwa cikin sauki daga kajin da suka kamu da ita zuwa lafiyayyun kaji ta hanyar abinci, ruwa, da haduwar jiki.
Ka'idar Gwajin
Kit ɗin yana amfani da hanyar ELISA mai gasa, riga-kafin cuta mai cutar bursal VP2 sunadaran furotin akan microplate, kuma yayi gasa tare da anti-VP2 protein antibody a cikin jini don ingantaccen vector mai ƙarfi ta amfani da anti-VP2 furotin monoclonal antibody.A cikin gwajin, an gwada maganin rigakafi na monoclonal kuma an ƙara furotin na anti-VP2, kuma bayan shiryawa, idan samfurin ya ƙunshi ƙwayar cutar bursal mai cutar kaji VP2 takamaiman antibody, yana ɗaure zuwa antigen akan farantin mai rufi.Ta haka toshe ɗaurin anti-VP2 protein monoclonal antigen zuwa antigen, bayan wankewa don cire maganin da ba a ɗaure ba da sauran abubuwan;sa'an nan kuma ƙara wani anti-mouse enzyme-labeled antibody na biyu don ɗaure musamman ga rukunin antigen-antibody akan farantin ganowa;Ana cire conjugate enzyme wanda ba a ɗaure ba ta hanyar wankewa;TMB substrate yana ƙara zuwa microwell don haɓaka launi, kuma ƙimar ƙimar samfurin tana da alaƙa da alaƙa da abun ciki na anti-VP2 antibody wanda ke ƙunshe a ciki, don haka cimma manufar gano anti-VP2 antibody antibody a cikin samfurin.
Abubuwan da ke ciki
| Reagent | Ƙarar Gwaje-gwaje 96/192 Gwaji | ||
| 1 |
| 1e/2 ku | |
| 2 |
| 2.0ml ku | |
| 3 |
| 1.6ml ku | |
| 4 |
| 100 ml | |
| 5 |
| 100 ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| ml 15 | |
| 9 |
| 2e/4 ku | |
| 10 | kwayoyin dilution microplate | 1e/2 ku | |
| 11 | Umarni | 1 inji mai kwakwalwa |
