Takaitawa | Gano takamaiman Antibody na Peste Des Petits Ruminants |
Ka'ida | Kayan gwajin PPRV na ELISA da ake amfani da shi don gano ƙwayoyin ƙwayoyin cuta na Peste des petits a cikin maganin tumaki da akuya. |
Abubuwan Ganewa | PPV antibody |
Misali | Magani
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Yawan | 1 kit = 192 Gwaji |
Kwanciyar hankali da Ajiya | 1) Duk reagents ya kamata a adana a 2 ~ 8 ℃.Kar a daskare. 2) Rayuwar rayuwa wata 12 ce.Yi amfani da duk reagents kafin ranar karewa akan kit.
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Ovine rinderpest, kuma akafi sani dapeste des dabbobin daji(PPR), cuta ce mai yaduwa da farko da ta fi shafaawakikumatumaki;duk da haka, raƙuma da ƙananan dajidabbobin dajikuma za a iya shafa.PPR a halin yanzu yana cikinArewa, Tsakiya, YammakumaGabashin Afirka, daGabas ta Tsakiya, kumaKudancin Asiya. Yana haifar da shikananan ruminants morbillivirusa cikin jinsiMorbillivirus,kuma yana da kusanci da, da sauransu. cutar morbillivirus, cutar kyanda morbillivirus, kumaciwon daji morbillivirus(da aka sani dacanineVirus distemper).Cutar tana da saurin yaduwa, kuma tana iya samun adadin mace-mace a cikin 80-100%.mlokuta a cikin waniepizooticsaitin.Kwayar cutar ba ta cutar da mutane.
PPR kuma ana kiranta da annoba ta awaki,kata, ciwo na stomatitis-pneumoenteritis, da kuma itacen ovine rinderpest.
Hukumomin hukuma irin suFAOkumaOIEamfani da sunan Faransa"peste des dabbobin daji" tare da bambance-bambancen rubutu da yawa.
Wannan kit ɗin yana amfani da hanyar ELISA gasa zuwa ga antigens na PPRV da aka riga aka rufawa akan rijiyoyin microplate.Lokacin gwaji, ƙara samfurin ruwan magani mai narkewa, bayan shiryawa, idan akwai maganin rigakafi na PPRV, zai haɗu tare da antigen da aka riga aka rufe, antibody a cikin samfurin yana toshe haɗin antigen monoclonal da riga-kafi;jefar da enzyme da ba a haɗa su ba tare da wankewa;Ƙara TMB a cikin ƙananan rijiyoyi, siginar shuɗi ta hanyar Enzyme catalysis yana cikin juzu'i na abun ciki na antibody a cikin samfurin.
Reagent | Ƙarar Gwaje-gwaje 96/192 Gwaji | ||
1 |
| 1e/2 ku | |
2 |
| 2ml ku | |
3 |
| 1.6ml ku | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 11/22 ml | |
7 |
| 11/22 ml | |
8 |
| ml 15 | |
9 |
| 2e/4 ku | |
10 | kwayoyin dilution microplate | 1e/2 ku | |
11 | Umarni | 1 inji mai kwakwalwa |