Kayayyaki
Cututtukan rigakafin cutar Newcastle ELISA Kit
Cututtukan rigakafin cutar Newcastle ELISA Kit
| Takaitawa | Gano takamaiman Antibody na Newcastle takamaiman ƙwayoyin rigakafi |
| Ka'ida | Ana amfani da kayan rigakafin cutar Newcastle Elisa kit don gano takamaiman rigakafin cutar ta Newcastle Kwayar cuta (NDV) a cikin jini, don sa ido kan antibody bayan rigakafi na NDVda kuma serological ganewar asali na kamuwa da cuta a cikin Avian.
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| Abubuwan Ganewa | Newcastle cutar antibody |
| Misali | Magani
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| Yawan | 1 kit = 192 Gwaji |
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Kwanciyar hankali da Ajiya | 1) Duk reagents ya kamata a adana a 2 ~ 8 ℃.Kar a daskare. 2) Rayuwar rayuwa wata 12 ce.Yi amfani da duk reagents kafin ranar karewa akan kit.
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Bayani
Cutar Newcastle cuta ce mai saurin yaduwa mai saurin yaduwa da ke shafar nau'ikan tsuntsayen gida da na daji;yana iya yadawa ga mutane.Ko da yake yana iya cutar da mutane, yawancin lokuta ba su da alamun bayyanar;da wuya yana iya haifar da zazzaɓi mai laushi da alamun mura kamar mura da/ko ciwon kai a cikin mutane.Abubuwan da ke haifar da shi sun fi shahara a cikin kaji na cikin gida saboda girman su da kuma yuwuwar tasirin tasirin epizootic akan masana'antar kiwon kaji.Yana da yaduwa a ƙasashe da yawa.Babu magani da aka sani, amma yin amfani da rigakafin rigakafi da matakan tsafta yana rage yiwuwar barkewar cutar.
Ka'idar Gwajin
Wannan kit ɗin yana amfani da hanyar toshe hanyar ELISA, NDV antigen an riga an rufe shi akan microplate.Lokacin gwaji, ƙara samfurin sinadarai mai diluted, bayan shiryawa, idan akwai takamaiman antibody na NDV, zai haɗu da antigen da aka rigaya, zubar da maganin da ba a haɗa shi da sauran abubuwan da aka haɗa tare da wankewa;sa'an nan ƙara enzyme mai lakabi anti-NDV monoclonal antibody, antibody a cikin samfurin toshe hade da monoclonal antibody da pre-rufi antigen;jefar da enzyme da ba a haɗa su ba tare da wankewa. Ƙara TMB a cikin ƙananan rijiyoyi, siginar shuɗi ta hanyar Enzyme catalysis yana cikin juzu'i na abun ciki na antibody a cikin samfurin.
Abubuwan da ke ciki
| Reagent | Ƙarar Gwaje-gwaje 96/192 Gwaji | ||
| 1 |
| 1e/2 ku | |
| 2 |
| 2.0ml ku | |
| 3 |
| 1.6ml ku | |
| 4 |
| 100 ml | |
| 5 |
| 100 ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| ml 15 | |
| 9 |
| 2e/4 ku | |
| 10 | kwayoyin dilution microplate | 1e/2 ku | |
| 11 | Umarni | 1 inji mai kwakwalwa |
