Kayayyaki
Nau'in Cutar Ƙafa da Baki O Ab ELISA Kayan Gwajin
Nau'in Cutar Ƙafa da Baki O Ab ELISA Kayan Gwajin
| Takaitawa | Gano takamaimanKafa da Baki antibodyNau'in O |
| Ka'ida | Nau'in FMD Nau'in Asiya I na gwajin gwajin ELISA da ake amfani da shi don gano ƙwayoyin ƙwayoyin cuta na ƙafa-da-baki a cikin maganin aladu, shanu, tumaki da akuya don kimanta rigakafin rigakafin FMD. |
| Abubuwan Ganewa | Kafa da Baki antibodyNau'in O |
| Misali | Magani
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| Yawan | 1 kit = 192 Gwaji |
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Kwanciyar hankali da Ajiya | 1) Duk reagents ya kamata a adana a 2 ~ 8 ℃. Kar a daskare. 2) Rayuwar rayuwa wata 12 ce. Yi amfani da duk reagents kafin ranar karewa akan kit.
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Bayani
Kwayar cuta ta ƙafa da baki(FMDV) shinepathogenwanda ke haddasawacutar ƙafa da baki.[1]Yana da apicornavirus, memba na jinsin halittuAphthovirus. Cutar, wanda ke haifar da vesicles (blisters) a cikin baki da ƙafa nashanu, aladu, tumaki, awaki, da sauran sumai kauri-kofatodabbobi suna da kamuwa da cuta sosai kuma babbar annoba cekiwon dabbobi
Ka'idar Gwajin
Wannan kituse-kai tsaye Hanyar ELISA, mai tsabta FMDVantigenispre-rubutu akan tsiri micro-rijiya enzyme. Lokacin gwaji, ƙarin samfurin serum, bayan shiryawa, idan akwai takamaiman rigakafin ƙwayoyin cuta na FMD, zai haɗu tare da antigen da aka riga aka rufe, a zubar da maganin da ba a haɗa shi da sauran abubuwan da aka gyara tare da wankewa; sa'an nan ƙara enzyme conjugate, jefar da uncombined enzyme conjugate tare da wanka. Ƙara TMB a cikin ƙananan rijiyoyi, siginar shuɗi ta hanyar Enzyme catalysis shine rabon abun ciki na antibody kai tsaye a cikin samfurin.
Abubuwan da ke ciki
| Reagent | Ƙarar Gwaje-gwaje 96/192 Gwaji | ||
| 1 |
| 1e/2 ku | |
| 2 |
| 2ml ku | |
| 3 |
| 1.6ml ku | |
| 4 |
| 100 ml | |
| 5 |
| 100 ml | |
| 6 |
| 11/22 ml | |
| 7 |
| 11/22 ml | |
| 8 |
| ml 15 | |
| 9 |
| 2e/4 ku | |
| 10 | kwayoyin dilution microplate | 1e/2 ku | |
| 11 | Umarni | 1 inji mai kwakwalwa |
